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ec growth medium  (PromoCell)


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    PromoCell ec growth medium
    Ec Growth Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/endothelial+cell+growth+medium+mv+kit/Endothelial+Cell+Growth+Medium+MV+Kit/pm42874163-96-6-10
    Average 95 stars, based on 46 article reviews
    ec growth medium - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Extraction:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Lysis:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Knock-Out:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Derivative Assay:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    In Vivo:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Recombinant:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    High Performance Liquid Chromatography:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Purification:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Permeability:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    In Vitro:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Membrane:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Saline:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Injection:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Labeling:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Standard Deviation:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Cell Culture:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Staining:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Expressing:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Western Blot:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Real-time Polymerase Chain Reaction:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Confocal Microscopy:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Double Staining:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Construct:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Activation Assay:

    Article Title: Endothelial SLC25A13 links glutamine metabolism to angiogenesis.
    Article Snippet: entific 11965092 M199 medium Thermo Fisher Scientific 11150059 Knock-out ™ DMEMmedium Thermo Fisher Scientific 10829018 EGM-2 Bullet kit Lonza CC-3202 RPMI 1640 GIBCO 11875093 Pericyte Growth Medium 2 PromoCell C-28041 ECGM-MV Kit PromoCell C-22120 CM-H2DCFDA Thermo Fisher Scientific C6827 dNTPs Thermo Fisher Scientific 10297018 Mevalonate Sigma-Aldrich 90469-50MG Cholesterol Sigma-Aldrich C4951-30

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.



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